International Standard
ISO/TS 20224-6:2020
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 6: Horse DNA detection method
Reference number
ISO/TS 20224-6:2020
Edición 1
2020-07
International Standard
Vista previa
ISO/TS 20224-6:2020
75479
No disponible en español
Publicado (Edición 1, 2020)

ISO/TS 20224-6:2020

ISO/TS 20224-6:2020
75479
Idioma
Formato
CHF 96
Convertir Franco suizo (CHF) a tu moneda

Resumen

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of horse-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of horse material derived from domestic horse (Equus caballus), mule (Equus caballus ♀ × Equus asinus ♂), hinny (Equus caballus ♂ × Equus asinus ♀) and zebroid (Equus caballus × Equus simplicidens). The assay also detects the species Przewalski's horse (Equus przewalskii) and zebra (Equus burchellii).

The target sequence is an Equus caballus isolate Twilight breed thoroughbred chromosome 28, EquCab3.0, whole genome shotgun sequence (i.e. GenBank accession number NC_009171.3)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).

Informaciones generales

  •  : Publicado
     : 2020-07
    : Cierre de la revisión [90.60]
  •  : 1
     : 15
  • ISO/TC 34/SC 16
    67.050 
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